Crosslinking Reagents
Thermo Scientific™ EZ-Link™ TFP Ester-PEG4-DBCO
An amine reactive heterobifunctional crosslinker which contains a dibenzylcyclooctyne (DBCO) group for copper-free click chemistry applications. The crosslinker can be used to label a variety of amine containing molecules such as proteins, biomolecules, or small molecules.
Thermo Scientific™ BMPS (N-β-maleimidopropyl-oxysuccinimide ester)
Crosslink amines to sulfhydryls with this very short amine-to-sulfhydryl crosslinker composed of NHS-ester and maleimide groups at ends of an aliphatic, 5-atom spacer arm.
Thermo Scientific™ Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate), No-Weigh™ Format
Crosslink amines to sulfhydryls with this mid-length, water-soluble crosslinker made of NHS-ester and maleimide groups at ends of a cyclohexane-containing, 8-atom spacer arm.
Invitrogen™ Succinimidyl 3-(2-Pyridyldithio)Propionate (SPDP)
The succinimidyl ester chemically reacts with primary amines and after mild reduction of the dithiol, it can react form a disulfide with other thiols.
Thermo Scientific™ SDA (NHS-Diazirine) (succinimidyl 4,4'-azipentanoate)
Very short to mid-length (3.9 to 13.5A), amine-to-anything crosslinkers; NHS-ester and diazirine reactive groups; photoactivate with UV light (Amax 330 to 370nm).
Thermo Scientific Pierce™ Pierce™ SM(PEG)12, No-Weigh™ Format
Thermo Scientific™ Pierce™ SM(PEG)12 is hetero-bifunctional crosslinker with N-hydroxysuccinimide (NHS) ester and maleimide groups that allow covalent conjugation of amine- and sulfhydryl-containing molecules.
Thermo Scientific™ Sulfo-SDA (Sulfo-NHS-Diazirine) (sulfosuccinimidyl 4,4'-azipentanoate)
Very short to mid-length (3.9 to 13.5A), amine-to-anything crosslinkers; NHS-ester and diazirine reactive groups; photoactivate with UV light (Amax 330 to 370nm).
Thermo Scientific™ EGS (ethylene glycol bis(succinimidyl succinate))
Crosslink amines to amines with this membrane permeable crosslinker composed of NHS-ester groups and a 16.1A hydroxylamine-cleavable spacer arm.
Thermo Scientific™ EZ-Link™ Maleimide-PEG4-DBCO
A sulfhydryl reactive heterobifunctional crosslinker which contains a dibenzylcyclooctyne (DBCO) group for copper-free click chemistry applications. The thiol/sulfhdryl reactive maleimide can be used to label a variety of thiol containing moieties such as free cystines in proteins, peptides, or other (bio)molecules.
Thermo Scientific™ SM(PEG)2 (PEGylated SMCC crosslinker)
Crosslink amines to sulfhydryls with these crosslinkers containing NHS-ester and maleimide groups at ends of water-soluble polyethylene glycol spacer arms (17.6 to 95.2A).
Thermo Scientific™ DSPP (Disuccinimidyl Phenyl Phosphonic Acid, PhoX), 50 mg PROMO
Improve mass spectrometry detection of crosslinked proteins and interactions with DSPP and TBDSPP heterotrifunctional crosslinkers.
Thermo Scientific™ TBDSPP (tert-Butyl Disuccinimidyl Phenyl Phosphonate, tBu-PhoX), 50 mg PROMO
Improve mass spectrometry detection of crosslinked proteins and interactions with DSPP and TBDSPP heterotrifunctional crosslinkers.
Thermo Scientific™ BS(PEG)9
Crosslink amines to amines with these long (21.7A and 35.8A) crosslinkers composed of NHS-ester groups and 5-unit or 9-unit PEG spacer arms that enhance protein solubility.
Thermo Scientific™ SM(PEG)8 (PEGylated, long-chain SMCC crosslinker)
Crosslink amines to sulfhydryls with these crosslinkers containing NHS-ester and maleimide groups at ends of water-soluble polyethylene glycol spacer arms (17.6 to 95.2A).