Crosslinking Reagents
Invitrogen™ Succinimidyl 3-(2-Pyridyldithio)Propionate (SPDP)
The succinimidyl ester chemically reacts with primary amines and after mild reduction of the dithiol, it can react form a disulfide with other thiols.
Thermo Scientific™ PMPI (p-maleimidophenyl isocyanate)
Short (8.7A) hydroxyl-to-sulfhydryl crosslinker; isocyanate and maleimide groups; label -OH by urethane bonds for conjugation to cysteines by thioether bonds.
Thermo Scientific™ Pierce™ DSP, No-Weigh™ Format
Crosslink amines to amines with dithiobis succinimidyl propionate, composed of NHS-ester groups and disulfide-containing, 12.0A spacer arm and cleavable with reducing agents.
Thermo Scientific™ AMAS (N-α-maleimidoacet-oxysuccinimide ester)
Crosslink amines to sulfhydryls with this very short crosslinker composed of NHS-ester and maleimide groups at ends of an aliphatic, 4-atom (4.4A) spacer arm.
Thermo Scientific™ Sulfo-EMCS (N-ε-maleimidocaproyl-oxysulfosuccinimide ester)
Crosslink amines to sulfhydryls with this mid-length crosslinker composed of sulfo-NHS-ester and maleimide groups at ends of a water-soluble, 8-atom (9.4A) spacer arm.
Thermo Scientific Pierce™ Pierce™ SM(PEG)2, No-Weigh™ Format
Thermo Scientific™ Pierce™ SM(PEG)2 is hetero-bifunctional crosslinker with N-hydroxysuccinimide (NHS) ester and maleimide groups that allow covalent conjugation of amine- and sulfhydryl-containing molecules.
Thermo Scientific™ DTME (dithiobismaleimidoethane)
Crosslink sulfhydryls to sulfhydryls (cysteine residues) with this long, cleavable crosslinker composed of maleimide groups and 11-atom (13.3A) disulfide spacer arm.
Thermo Scientific™ BS(PEG)5 (PEGylated bis(sulfosuccinimidyl)suberate)
Crosslink amines to amines with these long (21.7A and 35.8A) crosslinkers composed of NHS-ester groups and 5-unit or 9-unit PEG spacer arms that enhance protein solubility.
Thermo Scientific™ EZ-Link™ Maleimide-PEG4-DBCO
A sulfhydryl reactive heterobifunctional crosslinker which contains a dibenzylcyclooctyne (DBCO) group for copper-free click chemistry applications. The thiol/sulfhdryl reactive maleimide can be used to label a variety of thiol containing moieties such as free cystines in proteins, peptides, or other (bio)molecules.
Thermo Scientific™ Mass Spectrometry Compatible Crosslinkers
Improve mass spectrometry detection of crosslinked proteins and interactions with DSPP and TBDSPP heterotrifunctional crosslinkers.
Thermo Scientific™ Sulfo-EGS (ethylene glycol bis(sulfosuccinimidyl succinate))
Crosslink amines to amines with a crosslinker composed of sulfo-NHS-ester groups and hydroxylamine-cleavable spacer arm; water-soluble for cell-surface protein crosslinking.
Thermo Scientific™ LC-SPDP (succinimidyl 6-(3(2-pyridyldithio)propionamido)hexanoate)
Crosslink amines to sulfhydryls with this crosslinker composed of NHS-ester and pyridyldithiol groups at ends of 10-atom (15.7A) spacer arm.
Thermo Scientific™ BMH (bismaleimidohexane)
Crosslink sulfhydryls to sulfhydryls (cysteine residues in proteins or peptides) with this long crosslinker composed of maleimide groups and 11-atom (13.0A) spacer arm.
Thermo Scientific™ BM(PEG)2 (1,8-bismaleimido-diethyleneglycol)
Crosslink sulfhydryls to sulfhydryls (cysteine residues) with this long, pegylated, water-soluble crosslinker composed of maleimide groups and 2-unit (14.7A) PEG spacer arm.
Thermo Scientific™ SDAD (NHS-SS-Diazirine) (succinimidyl 2-((4,4'-azipentanamido)ethyl)-1,3'-dithiopropionate)
Very short to mid-length (3.9 to 13.5A), amine-to-anything crosslinkers; NHS-ester and diazirine reactive groups; photoactivate with UV light (Amax 330 to 370nm).